6th Edition of Neurology World Conference 2026

Abstract

Neurodegenerative diseases have started to attract attention due to their increasing prevalence day by day. Parkinson's Disease (PD) is today's second most common neurodegenerative disease. In the known pathology of Parkinson's disease, the loss of dopaminergic neurons in the substantia nigra of the brain occurs as a result of the accumulation of the (α)-synuclein component formed by the accumulation of Lewy bodies in specific genes and damaged neurons. The treatment and early diagnosis possibilities of Parkinson's disease, one of the most common neurodegenerative diseases, are minimal. For this reason, the condition needs to be investigated in more detail. There are in vitro and in vivo models in research. However, both types of research have their difficulties. Therefore, new models are needed. In this study, we aimed to develop a 3D culture medium with hydrogel-based bio-ink in neuron cells and then induce Parkinson's disease through neurotoxin. It was aimed to investigate the therapeutic efficacy of the nano-sized exosome-based formulation developed later. Then 2-Dimensional (2D) and 3-dimensional (3D) culture mediums were created with the dopaminergic neuroblastoma cell line (SH-SY5Y), and these culture mediums were induced into Parkinson's model. The therapeutic efficacy was investigated by Live&Dead analysis, processing these analysis images in the trainable Weka Program program, and finally, by immunostaining method. According to the results, The neuroprotective effect of dopamine-loaded exosomes has been proven in 2D and 3D culture mediums induced in the PD model. At the end of 1 week, the cells were determined to take a spheroidal form. Findings revealed that dopamine-loaded exosomes protect cells against 6-OHDA. Accordingly, we predict that exosome-based treatment methods will be a promising approach to treating Parkinson’s.